8AU0

Crystal structure of the a1 luminal coiled-coil domain of SUN1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-12
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	31.313, 35.986, 46.095
Unit cell angles	90.00, 104.54, 90.00

Refinement procedure

Resolution	30.310 - 2.070
R-factor	0.2444
Rwork	0.244
R-free	0.25510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Ideal helices
RMSD bond length	0.006
RMSD bond angle	0.788
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	Arcimboldo
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.310	2.105
High resolution limit [Å]	2.069	2.069
Rpim	0.043	0.287
Number of reflections	5986	294
<I/σ(I)>	8.6
Completeness [%]	96.6
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.09M Sodium nitrate, 0.09M Disodium phosphate, 0.09M Ammonium sulfate, 0.1M Imidazole pH 6.5, 0.1M MES (acid), 37.5% MPD (racemic), 37.5% PEG 1K, 37.5% PEG 3350