8ASU
The Crystal structure of short F46Y agroavidin - biotin complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-23 |
Detector | DECTRIS EIGER2 S 16M |
Wavelength(s) | 1.0723 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 81.365, 81.973, 68.123 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.090 - 2.520 |
R-factor | 0.20762 |
Rwork | 0.204 |
R-free | 0.27309 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8ass |
RMSD bond length | 0.007 |
RMSD bond angle | 1.727 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.750 | 2.590 |
High resolution limit [Å] | 2.520 | 2.520 |
Number of reflections | 15697 | 1118 |
<I/σ(I)> | 11.2 | |
Completeness [%] | 98.5 | 98.5 |
Redundancy | 4.6 | 4.7 |
CC(1/2) | 0.998 | 0.507 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 3350, 0.1 M Bis Tris |