8ASS
The Crystal structure of F46Y mutant of the agroavidin-biotin complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-23 |
Detector | DECTRIS EIGER2 S 16M |
Wavelength(s) | 0.972 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 80.138, 82.823, 67.472 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.800 - 2.120 |
R-factor | 0.24031 |
Rwork | 0.237 |
R-free | 0.31932 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8an6 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.632 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.800 | 2.190 |
High resolution limit [Å] | 2.120 | 2.120 |
Rmerge | 0.109 | 0.977 |
Number of reflections | 24821 | 2348 |
<I/σ(I)> | 7.3 | |
Completeness [%] | 95.5 | |
Redundancy | 4.2 | |
CC(1/2) | 0.997 | 0.492 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Bis Tris pH 5.8 20% PEG 3350 |