Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 270 |
| Detector technology | PIXEL |
| Collection date | 2022-08-03 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 2.00 |
| Spacegroup name | H 3 |
| Unit cell lengths | 64.490, 64.490, 46.190 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.590 - 2.955 |
| Rwork | 0.125 |
| R-free | 0.22980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | X3DNA |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.196 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.590 | 3.020 |
| High resolution limit [Å] | 2.950 | 2.970 |
| Number of reflections | 2783 | 1399 |
| <I/σ(I)> | 2 | |
| Completeness [%] | 97.0 | |
| Redundancy | 9.2 | |
| CC(1/2) | 0.992 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 20%MPD, 120mMMgCl2, 60mM NaCaCo, 2mM Spermine |
