8AS3
Structure of arrestin2 in complex with 6P CCR5 phosphopeptide and Fab30
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.999998 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 116.314, 121.064, 145.285 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.310 - 3.500 |
R-factor | 0.2869 |
Rwork | 0.284 |
R-free | 0.33260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jqi |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.1_3865) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.310 | 3.630 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.144 | 4.240 |
Rpim | 0.040 | 1.465 |
Number of reflections | 13152 | 1295 |
<I/σ(I)> | 13.2 | 0.7 |
Completeness [%] | 98.2 | 90.3 |
Redundancy | 13.6 | 13.6 |
CC(1/2) | 0.999 | 0.259 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1% tryptone (w/v), 0.05M HEPES, 12 % PEG3,350 |