8ARV

Structure of the EAL domain of BifA from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 2 21 21
Unit cell lengths	34.697, 117.503, 117.824
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.250 - 1.900
R-factor	0.2112
Rwork	0.209
R-free	0.24890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Modeller
RMSD bond length	0.005
RMSD bond angle	0.892
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.1_3865)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.270	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.123	1.600
Rmeas	0.133	1.700
Rpim	0.050	0.640
Number of reflections	38626	2434
<I/σ(I)>	14.8	2.3
Completeness [%]	99.1	97.9
Redundancy	13.1	13.6
CC(1/2)	0.998	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.4	291	0.1 M Tris pH 8.4, 36 % w/v PEG monomethyl ether 2,000