8ARU
Crystal Structure of human formylglycine generating enzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-01-19 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.92 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 43.497, 61.562, 109.330 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.880 - 1.080 |
R-factor | 0.142 |
Rwork | 0.140 |
R-free | 0.17360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1z70 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.500 | 40.880 | 1.140 |
High resolution limit [Å] | 1.080 | 4.960 | 1.110 |
Rmerge | 0.093 | 0.021 | 1.221 |
Rmeas | 0.107 | 0.023 | 1.537 |
Number of reflections | 114914 | 1428 | 7396 |
<I/σ(I)> | 10.12 | 46.6 | 0.69 |
Completeness [%] | 98.5 | 95.6 | 86.9 |
Redundancy | 4.089 | 4.125 | 2.203 |
CC(1/2) | 0.998 | 0.999 | 0.332 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 7-8 mg/mL protein in 20mM Tris/HCl pH8.0 mixed 1:1 with reservoir 0.1M Tris/HCl pH 8-9, 20-25% PEG4000, 0.25M CaCl2 |