8ARF
Crystal structure of the N-terminal parallel dimeric coiled-coil region of the human kinetochore associated protein Spindly
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.966000 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 112.507, 112.507, 49.642 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.720 - 2.800 |
Rwork | 0.249 |
R-free | 0.28980 |
Structure solution method | SIRAS |
RMSD bond length | 0.019 |
RMSD bond angle | 1.887 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | CRANK2 |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.720 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.079 | 1.558 |
Rpim | 0.050 | 0.970 |
Number of reflections | 9079 | 1312 |
<I/σ(I)> | 10.8 | 1.5 |
Completeness [%] | 99.3 | |
Redundancy | 5.4 | |
CC(1/2) | 0.999 | 0.718 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 300 | Bis-Tris Propane pH 6.5; 19% PEG3350; 0.2M KSCN |