8ARF

Crystal structure of the N-terminal parallel dimeric coiled-coil region of the human kinetochore associated protein Spindly

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.966000
Spacegroup name	P 32 2 1
Unit cell lengths	112.507, 112.507, 49.642
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.720 - 2.800
Rwork	0.249
R-free	0.28980
Structure solution method	SIRAS
RMSD bond length	0.019
RMSD bond angle	1.887
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.720	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.079	1.558
Rpim	0.050	0.970
Number of reflections	9079	1312
<I/σ(I)>	10.8	1.5
Completeness [%]	99.3
Redundancy	5.4
CC(1/2)	0.999	0.718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	300	Bis-Tris Propane pH 6.5; 19% PEG3350; 0.2M KSCN