8ARB
Heterologous Complex of shortened Aeromonas hydrophila Type III secretion substrate AscX with Yersinia enterocolitica chaperone YscY
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-07 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9919 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 90.330, 160.560, 156.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 80.280 - 2.630 |
R-factor | 0.293 |
Rwork | 0.291 |
R-free | 0.32620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7qih |
RMSD bond length | 0.002 |
RMSD bond angle | 0.358 |
Data reduction software | XDS (20200131) |
Data scaling software | XSCALE (20220220) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.280 | 2.700 |
High resolution limit [Å] | 2.630 | 2.630 |
Rmeas | 0.229 | 4.831 |
Number of reflections | 34253 | 2292 |
<I/σ(I)> | 9.3 | 0.61 |
Completeness [%] | 99.3 | 92.6 |
Redundancy | 24.86 | 12.93 |
CC(1/2) | 0.996 | 0.239 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | reservoir solution: 0.1 M Hepes pH 7.5, 0.6-0.9 M sodium dihydrogen phosphate, 0.6-0.9 M potassium dihydrogen phosphate [1.4-1.8 M total phosphate]; protein: 10 mg/mL in 20 mM Tris pH 8, 150 mM NaCl, 5 mM TCEP; drop size: 0.66 uL protein + 0.33 uL reservoir |