8AR8
Bovine glutamate dehydrogenase in complex with ADP at 2.4 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 1 |
Unit cell lengths | 87.510, 92.030, 119.570 |
Unit cell angles | 99.35, 106.73, 109.73 |
Refinement procedure
Resolution | 47.460 - 2.400 |
R-factor | 0.2052 |
Rwork | 0.204 |
R-free | 0.23610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3jcz |
RMSD bond length | 0.006 |
RMSD bond angle | 0.770 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.4 (8-JUN-2022)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.458 | 2.484 |
High resolution limit [Å] | 2.400 | 2.400 |
Rpim | 0.175 | 0.398 |
Number of reflections | 96056 | 4805 |
<I/σ(I)> | 5 | 2 |
Completeness [%] | 89.9 | 86 |
Redundancy | 3.4 | 3.6 |
CC(1/2) | 0.925 | 0.668 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20 mM CaCl2, 100 mM Na-acetate buffer (pH 4.6), 30% MPD (2-methyl-2,4-pentanediol) |