8AQD

Hydrophobic probe bound to Streptavidin - 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000413700316724
Spacegroup name	I 41 2 2
Unit cell lengths	57.581, 57.581, 183.943
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.030 - 1.450
R-factor	0.187
Rwork	0.184
R-free	0.23960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pk2
RMSD bond length	0.009
RMSD bond angle	1.502
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.030	1.470
High resolution limit [Å]	1.450	1.450
Number of reflections	28050	1360
<I/σ(I)>	15.4
Completeness [%]	100.0
Redundancy	20
CC(1/2)	0.997	0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293	25 w/v% PEG 3350 0.1 M sodium acetate pH 4.0