8AQ4
In surfo structure of the membrane integral lipoprotein N-acyltransferase Lnt from E. coli in complex with TITC and lyso-PE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.96862 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 160.111, 160.111, 90.934 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.130 - 2.620 |
| R-factor | 0.206 |
| Rwork | 0.205 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5n6h |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3600) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.130 | 3.010 |
| High resolution limit [Å] | 2.620 | 2.660 |
| Rmeas | 0.260 | 1.550 |
| Number of reflections | 24528 | 1226 |
| <I/σ(I)> | 8 | 1.7 |
| Completeness [%] | 93.2 | 60.8 |
| Redundancy | 9.8 | 9.2 |
| CC(1/2) | 0.990 | 0.530 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 50 mM sodium acetate pH 5.0, 50 mM magnesium acetate and 28-36 %(v/v) PEG200 |
