8AQ2

In meso structure of the membrane integral lipoprotein N-acyltransferase Lnt from P. aeruginosa covalently linked with TITC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	78.060, 116.230, 73.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.526 - 2.600
R-factor	0.2292
Rwork	0.228
R-free	0.25710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n6m
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_3494)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.526	2.700
High resolution limit [Å]	2.600	2.600
Rmeas	0.350	6.290
Number of reflections	21051	2200
<I/σ(I)>	6.93	0.85
Completeness [%]	99.7	99.7
Redundancy	14.63	14.59
CC(1/2)	0.990	0.350

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	30 %(v/v) PEG-500 DME, 0.1 M sodium citrate pH 5.0 and 0.1 M sodium acetate