8APX
CryoEM structure of the Chikungunya virus nsP1 capping pores in covalent complex with a 7GMP cap structure
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS TALOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Energy Filter | GIF Quantum LS |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.2 |
| Number of Particle | 111668 |
| Software | crYOLO,cryoSPARC,RELION,EPU,CTFFIND,Coot,PHENIX |
| Symmetry | C12 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
