8APL
Vaccinia virus DNA helicase D5 residues 323-785 hexamer with bound DNA processed in C6
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.1 |
| Number of Particle | 202659 |
| Software | RELION,CTFFIND,Coot,PHENIX |
| Symmetry | C6 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
