8API

THE S VARIANT OF HUMAN ALPHA1-ANTITRYPSIN, STRUCTURE AND IMPLICATIONS FOR FUNCTION AND METABOLISM

Replaces:  6API

Experimental procedure

Spacegroup name	P 65 2 2
Unit cell lengths	119.700, 119.700, 216.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	9999.000 * - 3.100
R-factor	0.215 *
Rwork	0.215
RMSD bond length	0.013
RMSD bond angle	2.400
Refinement software	EREF

Data quality characteristics

	Overall
High resolution limit [Å]	3.100 *
Number of reflections	15607 *
Completeness [%]	90.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8 *	4 *	Loebermann, H., (1982) Hoppe-Seyler'S Z.Physiol. Chem., 363, 1377. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium potassium phosphate	2.8 (M)
2	1	drop	sodium phosphate	5 (mM)
3	1	drop	protein	6-7 (mg/ml)