8AOQ
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with compound 20a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-10 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.660, 59.169, 88.334 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.160 - 2.088 |
Rwork | 0.195 |
R-free | 0.23250 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4v2y |
RMSD bond length | 0.007 |
RMSD bond angle | 1.463 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.200 | 2.210 |
High resolution limit [Å] | 2.088 | 2.090 |
Rmerge | 0.064 | 0.857 |
Number of reflections | 18215 | 5523 |
<I/σ(I)> | 17.04 | 2 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 7 | 6.96 |
CC(1/2) | 0.999 | 0.784 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.4M Ammonium Phosphate |