8ANW

Poliovirus type 3 (strain Saukett) stabilised virus-like particle (PV3 SC8).

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	FEI TITAN KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Energy Filter	GIF Quantum LS
Detector	GATAN K2 SUMMIT (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.7
Number of Particle	10744
Software	crYOLO,RELION,PHENIX,SerialEM,CTFFIND,UCSF Chimera
Symmetry	I
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION