8ANT
Crystal structure of 6xhis-tagged phi3T_93 protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97925 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.150, 50.828, 55.804 |
Unit cell angles | 90.00, 101.96, 90.00 |
Refinement procedure
Resolution | 37.200 - 1.900 |
R-factor | 0.2181 |
Rwork | 0.216 |
R-free | 0.24890 |
Structure solution method | AB INITIO PHASING |
RMSD bond angle | 5.690 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | Arcimboldo |
Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.830 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.081 | 0.382 |
Number of reflections | 16124 | 1017 |
<I/σ(I)> | 8 | |
Completeness [%] | 99.7 | 98.8 |
Redundancy | 6 | |
CC(1/2) | 0.995 | 0.975 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 294.15 | 0.1M Tris pH8, 5% glycerol, 0.6M Na/K tartrate |