8ANQ
Crystal structure of the microbial rhodopsin from Sphingomonas paucimobilis (SpaR)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.975997 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 233.339, 65.027, 124.689 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.910 - 2.800 |
| R-factor | 0.2761 |
| Rwork | 0.275 |
| R-free | 0.30330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c3w |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.082 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.320 | 46.320 | 2.910 |
| High resolution limit [Å] | 2.800 | 10.480 | 2.800 |
| Rmerge | 0.171 | 0.021 | 1.245 |
| Rmeas | 0.218 | 0.028 | 1.592 |
| Rpim | 0.133 | 0.018 | 0.980 |
| Total number of observations | 104259 | 1900 | 10595 |
| Number of reflections | 44606 | 865 | 4702 |
| <I/σ(I)> | 4.6 | 20 | 0.7 |
| Completeness [%] | 96.4 | 91.9 | 97.3 |
| Redundancy | 2.3 | 2.2 | 2.3 |
| CC(1/2) | 0.995 | 1.000 | 0.396 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 1.6M Ammonium Phosphate pH 5.2 |
