8ANO
Fucosylated mixed-chirality linear peptide FHP8 bound to the fucose binding lectin LecB PA-IIL from Pseudomonas aeruginosa at 1.3 Angstrom resolution.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-13 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.000023 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.805, 62.901, 64.565 |
| Unit cell angles | 90.00, 92.87, 90.00 |
Refinement procedure
| Resolution | 45.030 - 1.290 |
| R-factor | 0.1683 |
| Rwork | 0.167 |
| R-free | 0.20170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xoc |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.030 | 1.292 |
| High resolution limit [Å] | 1.285 | 1.285 |
| Rmeas | 0.099 | |
| Number of reflections | 207392 | 31662 |
| <I/σ(I)> | 8.08 | 0.81 |
| Completeness [%] | 98.2 | 92.7 |
| Redundancy | 3.22 | |
| CC(1/2) | 0.997 | 0.810 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.1 M HEPES pH 7.5, 25% w/v polyethylene glycol 3350 |
