8ANL
Structure of the amyloid-forming peptide LFIEWL from exendin-4, grown from water
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU PhotonJet-R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-08-24 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 1 |
| Unit cell lengths | 9.520, 14.292, 34.287 |
| Unit cell angles | 90.59, 93.30, 95.97 |
Refinement procedure
| Resolution | 14.210 - 1.750 |
| R-factor | 0.18 |
| Rwork | 0.176 |
| R-free | 0.21250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | crystal structure of LYIQWL |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.779 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.210 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmeas | 0.144 | 0.799 |
| Number of reflections | 1803 | 193 |
| <I/σ(I)> | 6.4 | 1.6 |
| Completeness [%] | 99.3 | 99.48 |
| Redundancy | 3.14 | |
| CC(1/2) | 0.984 | 0.700 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION, RECRYSTALLIZATION | 310 | Lyophilized peptide was dissolved in water (0.15 mg/ml concentration), followed by pH adjustment with NaOH to pH 5.50. Crystals appeared after incubation at 310K for several days. |
