8AN6
The Crystal structure of wt apo agroavidin - biotin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 80.089, 82.340, 67.314 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.720 - 1.900 |
| R-factor | 0.21773 |
| Rwork | 0.215 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Rhizavidin |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.549 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.720 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.084 | 0.978 |
| Number of reflections | 35567 | 2247 |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 3.9 | 4.1 |
| CC(1/2) | 0.990 | 0.640 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25% PEG 3350, 0.1 M MgCl2 and 0.1 M Bis Tris pH 6.0 |
