8AMR
Coiled-coil domain of human TRIM3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-21 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.9655 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 167.900, 167.900, 127.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.530 - 3.800 |
R-factor | 0.2749 |
Rwork | 0.273 |
R-free | 0.32130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | AlphaFold model of TRIM3 coiled coil |
RMSD bond length | 0.002 |
RMSD bond angle | 0.421 |
Data reduction software | XDS (Jan 10, 2022) |
Data scaling software | XDS (Jan 10, 2022) |
Phasing software | PHENIX (1.20.1_4487) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.530 | 3.840 |
High resolution limit [Å] | 3.710 | 3.710 |
Rmerge | 0.102 | 2.999 |
Number of reflections | 19774 | 1921 |
<I/σ(I)> | 19.26 | 1.15 |
Completeness [%] | 99.6 | |
Redundancy | 25.6 | |
CC(1/2) | 1.000 | 0.586 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 280.15 | 0.2 M potassium thiocyanate, 20% (w/v) PEG 3350 |