8AMJ
Crystal structure of unliganded AUGUGGCAU duplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.3 |
Synchrotron site | BESSY |
Beamline | 14.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-01-28 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.89429 |
Spacegroup name | H 3 |
Unit cell lengths | 49.124, 49.124, 69.729 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.970 - 2.020 |
R-factor | 0.2015 |
Rwork | 0.197 |
R-free | 0.24280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8ami |
RMSD bond length | 0.007 |
RMSD bond angle | 0.920 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.970 | 2.140 |
High resolution limit [Å] | 2.020 | 2.020 |
Rmerge | 0.061 | 0.722 |
Number of reflections | 4105 | 653 |
<I/σ(I)> | 16.78 | 1.88 |
Completeness [%] | 99.8 | |
Redundancy | 5.6 | |
CC(1/2) | 0.999 | 0.737 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 292 | 10mM AcOMg 50mM MES pH5.6 2.5M AS |