8ALM
X-ray structure of human NCS-1 bound to Ric-8A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-15 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979263 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 56.639, 56.639, 134.532 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.200 - 1.854 |
R-factor | 0.2118 |
Rwork | 0.210 |
R-free | 0.25250 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.011 |
RMSD bond angle | 1.136 |
Data reduction software | AutoProcess (1.0.5) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.201 | 1.942 |
High resolution limit [Å] | 1.854 | 1.854 |
Number of reflections | 17666 | 881 |
<I/σ(I)> | 15.2 | |
Completeness [%] | 91.1 | |
Redundancy | 8.7 | |
CC(1/2) | 0.999 | 0.467 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 30 % PEG 4000, 100 mM sodium acetate pH 4.6 |