8AKH

Crystal structure of DltE from L. plantarum soaked with LTA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	48.678, 96.066, 150.828
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.030 - 1.400
R-factor	0.1748
Rwork	0.175
R-free	0.19310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8agr
RMSD bond length	0.009
RMSD bond angle	1.106
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.030	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.022	0.442
Number of reflections	139817	13770
<I/σ(I)>	16.61
Completeness [%]	100.0
Redundancy	2
CC(1/2)	1.000	0.678

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 3350, ammonium tartrate