8AK2

Drosophila melanogaster UNC89 Protein Kinase Domain 1 (apo)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-12
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.92
Spacegroup name	P 63 2 2
Unit cell lengths	95.424, 95.424, 164.767
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.210 - 1.750
R-factor	0.1749
Rwork	0.174
R-free	0.19590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gu4
RMSD bond length	0.007
RMSD bond angle	0.808
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.210	1.770
High resolution limit [Å]	1.750	1.750
Number of reflections	45374	1485
<I/σ(I)>	17.89
Completeness [%]	99.9
Redundancy	13.12
CC(1/2)	0.999	0.342

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	291.15	1M potassium sodium tartrate, 100 mM CHES pH 9.5, 200 mM lithium sulfate