8AJY
Ruminococcus flavefaciens Cohesin-Dockerin structure: dockerin from ScaH adaptor scaffoldin in complex with the cohesin from ScaE anchoring scaffoldin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-09 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.873127 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.810, 120.000, 65.950 |
| Unit cell angles | 90.00, 97.77, 90.00 |
Refinement procedure
| Resolution | 65.340 - 1.710 |
| R-factor | 0.1961 |
| Rwork | 0.194 |
| R-free | 0.23720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4iu2 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.878 |
| Data reduction software | autoPROC (1.1.7) |
| Data scaling software | autoPROC (1.1.7) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.344 | 1.743 |
| High resolution limit [Å] | 1.710 | 1.714 |
| Rmerge | 0.108 | 0.500 |
| Rmeas | 0.124 | 0.590 |
| Rpim | 0.061 | 0.308 |
| Number of reflections | 66981 | 3283 |
| <I/σ(I)> | 8 | 2.1 |
| Completeness [%] | 99.6 | 99.3 |
| Redundancy | 3.8 | 3.4 |
| CC(1/2) | 0.994 | 0.789 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Lithium sulfate monohydrate, 0.1 M TRIS hydrochloride pH 8.5 and 30% w/v Polyethylene glycol 4000 |
