8AJI
Crystal structure of DltE from L. plantarum, TCEP form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-18 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.873130 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.236, 98.642, 135.934 |
| Unit cell angles | 90.00, 105.80, 90.00 |
Refinement procedure
| Resolution | 49.501 - 1.940 |
| Rwork | 0.183 |
| R-free | 0.21240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8agr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.397 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.501 | 1.970 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.095 | 0.395 |
| Number of reflections | 139757 | 6771 |
| <I/σ(I)> | 5.3 | |
| Completeness [%] | 97.7 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.988 | 0.811 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 3350, ammonium tartrate, TCEP HCl |
