8AIL

Bacillus phage VMY22 p56 in complex with Bacillus weidmannii Ung

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	289.15
Detector technology	PIXEL
Collection date	2017-04-21
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9762
Spacegroup name	P 1 21 1
Unit cell lengths	85.327, 97.495, 100.555
Unit cell angles	90.00, 111.36, 90.00

Refinement procedure

Resolution	48.870 - 2.450
Rwork	0.204
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4l5n
RMSD bond length	0.007
RMSD bond angle	1.413
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.870	2.520
High resolution limit [Å]	2.450	2.450
Number of reflections	56337	4587
<I/σ(I)>	2.53
Completeness [%]	99.8
Redundancy	4.4
CC(1/2)	0.993	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.2 M Sodium iodide, 0.1M Bis-tris propane pH 6.5, 20% PEG 3350