8AIF

TapA acts as specific chaperone in TasA non-amyloid filament formation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.91841
Spacegroup name	P 21 21 21
Unit cell lengths	39.199, 52.936, 167.763
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.440 - 1.070
R-factor	0.1486
Rwork	0.148
R-free	0.17660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6hqc
RMSD bond length	0.012
RMSD bond angle	1.655
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.440	1.130
High resolution limit [Å]	1.070	1.070
Rmeas	0.066	0.154
Number of reflections	153127	24261
<I/σ(I)>	9.77
Completeness [%]	98.8
Redundancy	3.53
CC(1/2)	0.999	0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	24% PEG3350, 0.35M LiNO3