8AHZ

Native VirD of Streptomyces virginiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-09-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.953709
Spacegroup name	P 41 21 2
Unit cell lengths	84.310, 84.310, 230.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.780 - 1.700
R-factor	0.1602
Rwork	0.159
R-free	0.17980
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.517
Data reduction software	XDS
Data scaling software	Aimless (0.5.29)
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.620	2.100	1.810
High resolution limit [Å]	1.700	2.000	1.760
Rmerge	0.071	0.030	0.753
Rmeas	0.074	0.032	0.788
Rpim	0.021	0.015	0.226
Number of reflections	87602	1110	5171
<I/σ(I)>	19.5
Completeness [%]	99.0	99.9	85.9
Redundancy	12.6	10.2	11.6
CC(1/2)	0.997	0.999	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	20% PEG 400, 20% PEG 800, 100 mM Tris-HCl