8AHR
Crystal structure of D-amino acid aminotransferase from Aminobacterium colombiense in holo form with PLP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.190, 80.974, 98.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.780 - 1.900 |
| R-factor | 0.1921 |
| Rwork | 0.189 |
| R-free | 0.24460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mqz |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.975 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.780 | 43.770 | 1.940 |
| High resolution limit [Å] | 1.900 | 9.110 | 1.900 |
| Rmerge | 0.081 | 0.030 | 0.754 |
| Rmeas | 0.094 | 0.035 | 0.870 |
| Rpim | 0.047 | 0.017 | 0.420 |
| Total number of observations | 1294 | 9896 | |
| Number of reflections | 38455 | 354 | 2489 |
| <I/σ(I)> | 11 | 33.4 | 1.9 |
| Completeness [%] | 98.1 | 89 | 99.3 |
| Redundancy | 3.9 | 3.7 | 4 |
| CC(1/2) | 0.998 | 0.999 | 0.614 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.2 M Sodium nitrate, 0.1 M Bis-tris propane pH 6.5, 20% PEG 3350 |
