8AHQ
VirD/holo-ACP5b of Streptomyces virginiae complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2017-09-29 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.95370 |
Spacegroup name | H 3 |
Unit cell lengths | 144.878, 144.878, 88.781 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.850 - 2.100 |
R-factor | 0.1856 |
Rwork | 0.183 |
R-free | 0.23760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | native |
RMSD bond length | 0.012 |
RMSD bond angle | 1.789 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 41.850 | 36.230 | 2.160 |
High resolution limit [Å] | 2.100 | 8.910 | 2.100 |
Rmerge | 0.125 | 0.049 | 1.467 |
Rmeas | 0.131 | 0.051 | 1.000 |
Rpim | 0.037 | 0.015 | 0.452 |
Number of reflections | 40503 | 520 | 3364 |
<I/σ(I)> | 15.5 | ||
Completeness [%] | 100.0 | 98.6 | 100 |
Redundancy | 11.8 | 12 | 11.1 |
CC(1/2) | 0.998 | 0.996 | 0.897 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM chloride calcium, 30% PEG 1500, 10% 2-propanol, 100 mM imidazole-HCl |