8AHN
Sin Nombre virus Gn in complex with Fab SNV-42
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-09 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 88.926, 146.420, 157.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.620 - 1.800 |
R-factor | 0.1911 |
Rwork | 0.190 |
R-free | 0.22130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 5OPG and PDB ID 5UR8 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.998 |
Data reduction software | xia2 (3.8.0-g3d57088-dials-3.8) |
Data scaling software | xia2 (3.8.0-g3d57088-dials-3.8) |
Phasing software | PHENIX (1.19.2_4158) |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.620 | 46.640 | 1.830 |
High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
Rmerge | 0.120 | ||
Rmeas | 0.125 | 0.056 | 1.434 |
Rpim | 0.033 | 0.015 | 0.379 |
Number of reflections | 95210 | 5023 | 4682 |
<I/σ(I)> | 13.4 | 50.4 | 1.1 |
Completeness [%] | 100.0 | 100 | 99.7 |
Redundancy | 13.8 | 14 | 14 |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 294 | 0.2 M tri-ammonium citrate pH 7.0 and 20% w/v polyethylene glycol 3350 |