8AHB
rsEGFP2 photoswitched to its off-state at room temperature and back-switched to its on-state at 100K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.668, 61.759, 70.445 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.440 - 1.790 |
| R-factor | 0.1934 |
| Rwork | 0.192 |
| R-free | 0.21960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dty |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.919 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP (11.7.03) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.440 | 1.858 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.127 | |
| Rpim | 0.038 | |
| Number of reflections | 254080 | 14673 |
| <I/σ(I)> | 10.87 | 0.45 |
| Completeness [%] | 99.4 | 94.43 |
| Redundancy | 11.8 | 7.3 |
| CC(1/2) | 0.999 | 0.237 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 293 | 100 mM HEPES buffer, pH 8.1 1.9 M ammonium sulfate |
