8AHA

rsEGFP2 photoswitched to its off-state at 100K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-03
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 21 21 21
Unit cell lengths	52.400, 60.755, 71.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.320 - 2.380
R-factor	0.1882
Rwork	0.186
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dtx
RMSD bond length	0.008
RMSD bond angle	0.999
Data reduction software	autoPROC (1.1.7)
Data scaling software	autoPROC (1.1.7)
Phasing software	MOLREP (11.7.03)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.320	2.465
High resolution limit [Å]	2.380	2.380
Rpim	0.071	0.408
Number of reflections	124380	12739
<I/σ(I)>	11.44	2.23
Completeness [%]	99.9	100
Redundancy	13	13.5
CC(1/2)	0.996	0.714

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.1	293	100 mM HEPES buffer, pH 8.1 1.9 M ammonium sulfate