8AGR
Crystal structure of DltE from L. plantarum, apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-18 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.8731 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.874, 95.719, 68.013 |
| Unit cell angles | 90.00, 96.26, 90.00 |
Refinement procedure
| Resolution | 47.796 - 1.860 |
| Rwork | 0.166 |
| R-free | 0.20270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wwx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.353 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.860 | 1.900 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rpim | 0.120 | 0.441 |
| Number of reflections | 64951 | 39993 |
| <I/σ(I)> | 6.4 | 2 |
| Completeness [%] | 99.9 | |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG MME 5K, Tris pH8.5, LiSO4, ethylene glycol |
