8AGA
Structure of p-hydroxy benzoic acid ligand bound HosA transcriptional regulator from enteropathogenic Escherichia coli O127:H6 (strain E2348/69)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2022-05-20 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54179 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 67.380, 67.380, 95.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.640 - 2.210 |
R-factor | 0.2086 |
Rwork | 0.205 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7Z4P |
RMSD bond length | 0.002 |
RMSD bond angle | 0.474 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487: ???) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.970 | 2.330 |
High resolution limit [Å] | 2.210 | 2.210 |
Number of reflections | 11485 | 1612 |
<I/σ(I)> | 18.7 | |
Completeness [%] | 99.8 | 98.4 |
Redundancy | 7.3 | 6.8 |
CC(1/2) | 0.998 | 0.946 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 277.15 | Potassium Chloride, Calcium Chloride, Sodium Cacodylate, PEG4000, pH6 |