8AG8

Structure of the Fluorescence Recovery-like protein FRPL from Pseudomonas borbori

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97625
Spacegroup name	P 43 21 2
Unit cell lengths	53.460, 53.460, 92.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 1.800
R-factor	0.2277
Rwork	0.227
R-free	0.23170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tz0
RMSD bond length	0.020
RMSD bond angle	1.461
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.334	1.864
High resolution limit [Å]	1.800	1.800
Number of reflections	13026	1220
<I/σ(I)>	32.02
Completeness [%]	98.2
Redundancy	22.2
CC(1/2)	1.000	0.960

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	293.15	0.2 M LiSO4, 0.1 M CHES pH 9.5, and 1.4 M Sodium/Potassium Tartrate.