8AG8
Structure of the Fluorescence Recovery-like protein FRPL from Pseudomonas borbori
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-08-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 53.460, 53.460, 92.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.000 - 1.800 |
R-factor | 0.2277 |
Rwork | 0.227 |
R-free | 0.23170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5tz0 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.461 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.334 | 1.864 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 13026 | 1220 |
<I/σ(I)> | 32.02 | |
Completeness [%] | 98.2 | |
Redundancy | 22.2 | |
CC(1/2) | 1.000 | 0.960 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293.15 | 0.2 M LiSO4, 0.1 M CHES pH 9.5, and 1.4 M Sodium/Potassium Tartrate. |