8AFP
Structure of fibronectin 2 and 3 of L1CAM at 3.0 Angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-14 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9762 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 55.477, 96.666, 106.578 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.800 - 3.000 |
R-factor | 0.2404 |
Rwork | 0.240 |
R-free | 0.25770 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.236 |
Data reduction software | XDS |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.250 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmeas | 0.160 | 1.000 |
Number of reflections | 11342 | 548 |
<I/σ(I)> | 12.1 | |
Completeness [%] | 97.3 | |
Redundancy | 13.9 | |
CC(1/2) | 0.990 | 0.980 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 150 mM sodium citrate pH 5.5, 10.6% PEG 20.000, 10 mM sodium potassium tartrate |