8AF9
Structure of a T4SS effector from Brucella sp.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-26 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97242 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 133.909, 133.909, 389.511 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.610 - 2.500 |
R-factor | 0.2174 |
Rwork | 0.216 |
R-free | 0.24480 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | AutoSol |
Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.690 | 48.690 | 2.550 |
High resolution limit [Å] | 2.500 | 12.250 | 2.500 |
Rmerge | 0.184 | 0.107 | 1.234 |
Rmeas | 0.189 | 0.112 | 1.273 |
Rpim | 0.043 | 0.033 | 0.310 |
Total number of observations | 1420281 | 11765 | 73144 |
Number of reflections | 72439 | 760 | 4384 |
<I/σ(I)> | 12.8 | 20.7 | 2.6 |
Completeness [%] | 100.0 | 98.4 | 100 |
Redundancy | 19.6 | 15.5 | 16.7 |
CC(1/2) | 0.993 | 0.992 | 0.927 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 25% PEG4000 0.2M CaCl2 100 mM Tris pH 8 |