8AEW
Malonyl-CoA reductase from Chloroflexus aurantiacus - N-terminal Apo
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.92819 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.761, 86.787, 260.489 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 82.340 - 2.720 |
R-factor | 0.2462 |
Rwork | 0.244 |
R-free | 0.29040 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.122 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | CRANK2 |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 82.340 | 2.817 |
High resolution limit [Å] | 2.720 | 2.720 |
Rmerge | 0.110 | 1.273 |
Rmeas | 0.155 | 1.000 |
Rpim | 0.110 | 1.000 |
Number of reflections | 68074 | 6553 |
<I/σ(I)> | 7.5 | |
Completeness [%] | 98.5 | 96.09 |
Redundancy | 2 | 2 |
CC(1/2) | 0.993 | 0.478 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M Sodium cacodylate, pH 6.5, 40% (w/v) MPD 5% (w/v) PEG 8000 |