8AEH
X-ray structure of Canis familiaris Odorant Binding Protein 1
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.978565 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 88.221, 88.221, 37.517 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.110 - 3.000 |
R-factor | 0.286 |
Rwork | 0.285 |
R-free | 0.30110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3eyc |
RMSD bond length | 0.010 |
RMSD bond angle | 1.466 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.110 | 3.180 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.066 | 0.188 |
Rmeas | 0.067 | 0.192 |
Rpim | 0.014 | 0.038 |
Number of reflections | 3242 | 512 |
<I/σ(I)> | 32.5 | 14.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 25 | |
CC(1/2) | 0.990 | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 4000, 0.2 M CaCl2 in 0.1 M pH 8.5 Tris buffer |