8ADK
Poly(ADP-ribose) glycohydrolase (PARG) from Drosophila melanogaster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97958 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.433, 115.622, 122.913 |
| Unit cell angles | 90.00, 112.21, 90.00 |
Refinement procedure
| Resolution | 59.025 - 2.474 |
| Rwork | 0.177 |
| R-free | 0.22770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6hmk |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.895 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.025 | 2.520 |
| High resolution limit [Å] | 2.458 | 2.460 |
| Rmerge | 0.110 | 1.618 |
| Rpim | 0.046 | 0.673 |
| Number of reflections | 88137 | 6515 |
| <I/σ(I)> | 9.7 | 1.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.7 | 6.7 |
| CC(1/2) | 0.996 | 0.579 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 277.15 | 19% (v/v) PEG3350, 210 mM Na2SO4, 100 mM BisTris propane pH 7.2 |
