8ADC
Viral tegument-like DUBs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-08-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.121, 70.295, 75.890 |
| Unit cell angles | 90.00, 101.84, 90.00 |
Refinement procedure
| Resolution | 51.060 - 1.700 |
| R-factor | 0.1993 |
| Rwork | 0.198 |
| R-free | 0.23510 |
| Structure solution method | SIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.836 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.20_4478) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.079 | 1.180 |
| Rmeas | 0.080 | 1.410 |
| Number of reflections | 81659 | 13147 |
| <I/σ(I)> | 8.5 | 1 |
| Completeness [%] | 98.8 | 98.3 |
| Redundancy | 3.4 | 3.1 |
| CC(1/2) | 0.997 | 0.535 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.2 M sodium chloride, 0.1 M BisTris pH 5.5, 25 % w/v PEG 3350 |
