8ADB

Viral tegument-like DUBs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 32 2 1
Unit cell lengths	101.766, 101.766, 63.204
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	51.360 - 1.730
R-factor	0.1684
Rwork	0.167
R-free	0.19280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	WC-VDT1 1ubb
RMSD bond length	0.011
RMSD bond angle	1.216
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20_4478)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	1.840
High resolution limit [Å]	1.730	1.730
Rmerge	0.151	2.820
Rmeas	0.153
Number of reflections	39677	6346
<I/σ(I)>	11.4	1.1
Completeness [%]	100.0	100
Redundancy	19.6	19.2
CC(1/2)	0.998	0.497

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.1 M BisTris propane pH 7.0, 2.0 M Ammonium citrate tribasic