8ADA
Crystal structure of ClpC2 N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-27 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 48.140, 48.140, 110.018 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.690 - 1.996 |
| R-factor | 0.1814 |
| Rwork | 0.180 |
| R-free | 0.20950 |
| Structure solution method | AB INITIO PHASING |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | Arcimboldo |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 41.690 | 41.690 | 2.050 |
| High resolution limit [Å] | 1.996 | 8.930 | 2.000 |
| Rmerge | 0.100 | 0.050 | 1.189 |
| Rmeas | 0.106 | 0.054 | 1.260 |
| Rpim | 0.034 | 0.020 | 0.408 |
| Total number of observations | 1118 | 6022 | |
| Number of reflections | 10183 | 141 | 679 |
| <I/σ(I)> | 14 | 34.3 | 2.1 |
| Completeness [%] | 99.6 | 99.8 | 94.1 |
| Redundancy | 9.6 | 7.9 | 8.9 |
| CC(1/2) | 0.997 | 0.994 | 0.695 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.15 | 25 % w/v PEG 3350, 0.1 M Tris pH 8.5 |
