8AD9

Crystal structure of ClpC2 C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	C 1 2 1
Unit cell lengths	142.582, 40.455, 59.741
Unit cell angles	90.00, 112.47, 90.00

Refinement procedure

Resolution	38.670 - 1.430
R-factor	0.1817
Rwork	0.180
R-free	0.21090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wdc
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.670	38.670	1.450
High resolution limit [Å]	1.430	7.830	1.430
Rmerge	0.057	0.058	0.924
Rmeas	0.062	0.065	1.003
Rpim	0.024	0.028	0.384
Total number of observations	393155	2120	18785
Number of reflections	57672	385	2843
<I/σ(I)>	14.9	33.8	2.1
Completeness [%]	98.6	98.1	97.9
Redundancy	6.8	5.5	6.6
CC(1/2)	0.998	0.990	0.762

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293.15	100 mM C2H3NaO2 pH 4.5, 30 % (w/v) PEG 8K, 200 mM LiSO4